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F12培養(yǎng)基 Gibco試劑
簡(jiǎn)要描述：F12培養(yǎng)基 Gibco試劑英文名稱：Ham's F-12 Nutrient Mix, powder規(guī)格：10 x 1 L性狀：干粉培養(yǎng)基貨號(hào)：21700-075
產(chǎn)品型號(hào)： 21700-075
所屬分類：Gibco干粉培養(yǎng)基
更新時(shí)間：2025-02-19
廠商性質(zhì)：經(jīng)銷商
F12培養(yǎng)基 Gibco試劑 原裝正品，質(zhì)量保證，現(xiàn)貨供應(yīng) 450元/盒
英文名稱：Ham's F-12 Nutrient Mix, powder
規(guī)格：10 x 1 L
性狀：干粉培養(yǎng)基
貨號(hào)：21700-075
F12培養(yǎng)基 Gibco試劑 產(chǎn)品描述：
描述
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. Life Technologies offers a variety of Gibco® F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
| With | Without |
| • L-glutamine | • Sodium Bicarbonate |
| • Phenol Red |
The complete formulation is available.
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco® F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. In addition, the New York facility has ISO 9001 certification.
Gibco® F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
產(chǎn)品規(guī)格：
| Form: | Powder |
|---|---|
| Glutamine: | L-Glutamine |
| HEPES Buffer: | No HEPES |
| Product Size: | 10 x 1 L |
| Phenol Red Indicator: | Phenol Red |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Shipping Condition: | Room Temperature |
培養(yǎng)基配方
21700 - Ham's F-12, powder
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